Comparison of flow cytometry and Western blotting to measure Hsp70
نویسندگان
چکیده
منابع مشابه
Comparison of cell-ELISA, flow cytometry and Western blotting for the detection of antiendothelial cell antibodies.
OBJECTIVE There is still great uncertainty in the detection of antiendothelial cell antibodies (AECA). The aim of our study was to compare the results obtained using different methods. METHODS Sera were obtained from 71 patients with a variety of vasculitides. Three assay methods were used: cell ELISA, flow cytometry (FACS) and Western blot (WB). RESULTS In the ELISA 12/17 patients with sys...
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15 صفحه اولWestern Blotting
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متن کاملImmunoprecipitation and western blotting
SKG or BALB/c T cells (3.0 £ 10) were stimulated for 72 h with plate-bound anti-CD3 mAb (2C11) in the presence or absence of plate-bound anti-CD28 mAb in RPMI-1640 medium supplemented with 10% fetal calf serum and 50 mM 2-mercaptoethanol. Cells were also stimulated with TPA (1.4 ng ml) and ionomycin (0.14 mM). KJ1.26þ T cells from DO or DO.SKG mice were stimulated with ovalbumin (323–339) pepti...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1996
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19961101)25:3<280::aid-cyto9>3.3.co;2-q